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California, United States
Onsite
E11 Bio is on a mission to advance connectomics towards whole brain scale for humans and other mammals. We are a collaborative and interdisciplinary team of scientists and engineers engaged in a neuroscience moonshot project to develop a radical technology platform for scalable mammalian brain mapping. E11 Bio is a non-profit Convergent Research focused research organization located in Alameda, California, pursuing blue-skies neuroscience research yet operating like a nimble, tight-knit start-up. Read our technology roadmap.
E11 Bio is seeking a Research Associate to run the in vivo work that produces every sample the platform depends on. In collaboration with the team, you will run colony management, viral injections and perfusions, and grow into imaging and histology of downstream work. The role is open to early-career applicants as well as experienced technicians, and level and compensation are set by experience.
Execute retro-orbital and stereotaxic viral injections, with post-operative monitoring
Prepare perfusates and run perfusions to SOP standards, and keep perfusion and surgical setups operational
Collect and process tissue, including sectioning of the fixed brain, histology and immunostaining, and imaging
Run colony management and animal supply: breeding, weaning, genotyping workflows, health checks and record keeping
Support perfusion development: run controlled perturbations and document QC outcomes
Prepare equipment and supplies for experiments, keep the reagents and consumables for these workflows stocked, and flag supply problems early
Work to approved animal protocols and comply with federal and institutional IACUC regulations governing animal research
Keep accurate, reproducible experimental records
Completed training in retro-orbital and stereotaxic injections, and running routine surgeries with good survival and recovery
Performing perfusions under supervision at the team's baseline quality level, with perfusates prepared independently
Running the colony: health checks, weaning and genotyping workflows on schedule
Trained on imaging and running it routinely
Independently producing fixed tissue at baseline quality
Carrying one small focused piece of development work, scoped with a scientist
BS/BA in a STEM field with 0 to 4 years of relevant experience, or an MS, or equivalent hands-on experience
Experience with rodent handling, restraint and procedures under an approved protocol, and willingness to work with small animals and animal tissue
Strong lab notebook practice, careful experimental documentation and attention to detail
Ability to work some weekends as needed, with days off swapped for weekdays
Hands-on experience performing animal perfusions with PFA or related fixatives for brain tissue applications
Experience with stereotaxic or retro-orbital viral injections, or clear interest and aptitude to train into them
Experience with transgenic mouse colony management, including breeding and genotyping workflows
Experience with histology, brain excision or sectioning, and with acquiring images on a fluorescence microscope, or clear interest in moving into imaging and histology as part of the role
Mechanical or technical aptitude for maintaining and troubleshooting perfusion rigs and surgical setups
Are motivated by moonshot neuroscience and want to work on a high-impact project with a tight-knit team
Are committed to open science and want to see the work disseminated transparently
An opportunity to help change the world and work with some of the most talented and collaborative experts across scientific fields.
An opportunity to gain experience on a field-defining moonshot effort.
Excellent medical, dental, and vision insurance through a PPO plan; parental leave.
Generous time off + paid holidays.
401K matching up to 6% of base salary
Ample healthy and energizing food, including daily lunches.
Stipend for fitness and wellness activities.